Caenorhabditis briggsae Research Resource
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List of C. briggsae polymorphisms

(contributed by various labs)


cb-egl-5 Polymorphisms (Scott Baird Lab)

   Forward primer: 5'-CAG GGA GCG GAC AAC TTC AAA GG-3'  (exon 1)
   Reverse primer: 5'-GGA CAC AGC CCA GGA TTA GCG AC-3'  (exon 2)

Predicted amplification product (AF16 sequence) = 1,723 bp


Strain PCR product
cb-egl-5(+) [AF16] ~1,700 bp
cb-egl-5(bdP1) [HK104, HK105, VT847] ~1,500 bp
cb-egl-5(bdP2) [PB800] ~1,400 bp



cb-mes-2 Insertion/Deletion (Scott Baird Lab)

   Forward primer: 5'-CAG GCG AAA AAT GCT CTG ACA AGT G -3'  
   Reverse primer: 5'-AAT CCG TTC CGT TGA TGG AGG C -3'  

Predicted amplification product (AF16 sequence) = 1,013 bp


Strain PCR product
cb-mes-2(+) [AF-16] ~990 bp
cb-mes-2(bdP4) [HK104] ~880 bp



cb-mes-4 SNP (Scott Baird Lab)

   Forward primer: 5'-CGG AAA TGC GGA AAA TCG G-3'  
   Reverse primer: 5'-CTC CTT GAA GAA GAA ATG GTG GC-3'  

Predicted amplification product (AF16 sequence) = 907 bp


Strain Dra I cut
cb-mes-4(+) [AF-16] None
cb-mes-4(bdP3) [HK104] 680 bp + 227 bp